شناسایی و استخراج هیدروکربن‌های چند حلقه‌ای آروماتیک از بافت ماهیان دودی و اثرات آن بر روی شاخص‌های کیفی، میکروبی و ترکیب اسیدهای چرب

Background and purpose: Cold-smoking is one of the most common ways to increase the shelf life of fish during storage. Materials and methods: This study aimed to extract and measure the polycyclic aromatic hydrocarbon (PAH) carcinogens, using high-pressure liquid chromatography (HPLC)-mass, fatty acids profile, nutritional value, and bacterial community in three widely-used species of smoked fishes [Rutilus frisii (Kutum), Liza aurata, and Hypophthalmichthys molitri] of Caspian Sea, Iran. Results: Amounts of high molecular weight (HMW)-PAHs, as indicator of carcinogenic components in smoke, showed concentrations of 0.100, 0.039 and 0.033 µg/kg in smoked Hypophthalmichthys molitrix, Liza aurata and Rutilus frisii, respectively (P < 0.05). The concentration of Benzo[a]Pyrene was calculated in Hypophthalmichthys molitrix and Rutilus frisii 0.001, and in Liza aurata 0.004 µg/kg (P < 0.05). The amounts of ω-3 in Hypophthalmichthys molitrix, Liza aurata and Rutilus frisii were 15.79%, 11.18% and 8.74%, respectively (P < 0.05). The Poleyen index amounts (EPA+DHA/C16) were 0.30% in, 0.13% and 0.14% in Hypophthalmichthys molitrix, Liza aurata and Rutilus frisii, respectively (P < 0.05). High correlation was considered between amounts of lipids and PAHs (R2 = 0.56). Number of anaerobic bacteria in smoked Hypophthalmichthys molitrix, Liza aurata and Rutilus frisii was 5.26, 5.15 and 5.18 log ufc/g, respectively and amounts of total coliform (TC) was recorded 5.21, 5.23 and 5.20 in them, respectively (P < 0.05). Conclusion: Contents of PAHs, quality indexes and bacterial community were at the accepted ranges and the suitable unsaturated fatty acids (UFA) and Polyen index were considerable in studied fish.